BD-PP0731

Cdk7 (phospho Thr170) Rabbit Polyclonal Antibody

CDK7; CAK; CAK1; CDKN7; MO15; STK1; Cyclin-dependent kinase 7; 39 kDa protein kinase; p39 Mo15; CDK-activating kinase 1; Cell division protein kinase 7; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subu

常规抗体

CDK7

Cyclin-dependent kinase 7

WB

Human,Mouse

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1022

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1022

P50613

http://www.uniprot.org/uniprotkb/P50613/entry

12572

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12572

Q03147

http://www.uniprot.org/uniprot/Q03147

The antiserum was produced against synthesized peptide derived from human CDK7 around the phosphorylation site of Thr170. AA range:136-185

Phospho-Cdk7 (T170) Polyclonal Antibody detects endogenous levels of Cdk7 protein only when phosphorylated at T170.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

40kD

-20°C/1 year

Polyclonal, Rabbit,IgG

cyclin dependent kinase 7(CDK7) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq, Jul 2008],

Ubiquitous.

Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Colocalizes with PRKCI in the cytoplasm and nucleus (PubMed:15695176). Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides (PubMed:19071173). .

Nucleotide excision repair;Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;

catalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Inactivated by phosphorylation.,function:Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.,PTM:Phosphorylation of Ser-164 during mitosis inactivates the enzyme.,PTM:Phosphorylation of Thr-170 is required for activity.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Associates primarily with cyclin H and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor. Interacts with PUF60.,tissue specificity:Ubiquitous.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.