BD-PP0707-PLC β3 (phospho Ser537) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PLC β3 (phospho Ser537) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0707

产品名称

PLC β3 (phospho Ser537) Rabbit Polyclonal Antibody

别名

PLCB3; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3

类别

常规抗体

基因名称

PLCB3

蛋白名称

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5331

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5331

Human Swissprot No.

Q01970

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q01970/entry

Mouse Swissprot No.

P51432

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P51432

Rat Swissprot No.

Q99JE6

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q99JE6

免疫原

The antiserum was produced against synthesized peptide derived from human PLC beta3 around the phosphorylation site of Ser537. AA range:503-552

特异性

Phospho-PLC β3 (S537) Polyclonal Antibody detects endogenous levels of PLC β3 protein only when phosphorylated at S537.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

预测分子量

150kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],

组织表达

Epithelium,Uterus,

细胞定位

Cytoplasm . Membrane . Nucleus . And particulate fractions. .

信号通路

Stem cell pathway; WNT;WNT-T CELL;β-Catenin; AMPK

功能

catalytic activity:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.,cofactor:Calcium.,function:The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PI-PLC X-box domain.,similarity:Contains 1 PI-PLC Y-box domain.,subunit:Interacts with SHANK2 (By similarity). Interacts with LPAR2.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using PLC beta3 （Phospho-Ser537） Antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using PLC beta3 （Phospho-Ser537） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with UV 15‘, using PLC beta3 （Phospho-Ser537） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-PLC β3 （S537） Polyclonal Antibody diluted at 1：500

Western Blot analysis of 293T cells using Phospho-PLC β3 （S537） Polyclonal Antibody diluted at 1：500