产品名称
Cdc25C (phospho Thr48) Rabbit Polyclonal Antibody
别名
CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C
蛋白名称
M-phase inducer phosphatase 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=995
Human Swissprot No.
P30307
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P30307/entry
Mouse Swissprot No.
P48967
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P48967
免疫原
The antiserum was produced against synthesized peptide derived from human CDC25C around the phosphorylation site of Thr48. AA range:14-63
特异性
Phospho-Cdc25C (T48) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at T48.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
cell division cycle 25C(CDC25C) Homo sapiens This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015],
组织表达
Colon carcinoma,Epithelium,Skin,Testis,
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;Progesterone-mediated oocyte maturation;
功能
catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,developmental stage:Expressed predominantly in G2 phase.,function:Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity.,PTM:Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,subunit:Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
