BD-PP0675-Troponin I-C (phospho Ser22/S23) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Troponin I-C (phospho Ser22/S23) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0675

产品名称

Troponin I-C (phospho Ser22/S23) Rabbit Polyclonal Antibody

别名

TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I

类别

常规抗体

基因名称

Tnni3

蛋白名称

Troponin I cardiac muscle

推荐应用

反应种属

Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Mouse Gene ID

21954

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21954

Rat Gene ID

29248

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29248

Rat Swissprot No.

P23693

Rat Swissprot Link

http://www.uniprot.org/uniprot/P23693

免疫原

The antiserum was produced against synthesized peptide derived from mouse TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54

特异性

Phospho-Troponin I-C (S22/S23) Polyclonal Antibody detects endogenous levels of Troponin I-C protein only when phosphorylated at S22/S23.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

28kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using TNNI3 （Phospho-Ser22+Ser23） Antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using TNNI3 （Phospho-Ser22+Ser23） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from mouse heart, using TNNI3 （Phospho-Ser22+Ser23） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of SH-SY5Y cells using Phospho-Troponin I-C （S22/S23） Polyclonal Antibody diluted at 1：1000