BD-PP0662-MYPT1 (phospho Thr853) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MYPT1 (phospho Thr853) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0662

产品名称

MYPT1 (phospho Thr853) Rabbit Polyclonal Antibody

别名

PPP1R12A; MBS; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit

类别

常规抗体

基因名称

PPP1R12A

蛋白名称

Protein phosphatase 1 regulatory subunit 12A

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4659

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4659

Human Swissprot No.

O14974

Human Swissprot Link

http://www.uniprot.org/uniprotkb/O14974/entry

Mouse Gene ID

17931

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17931

Mouse Swissprot No.

Q9DBR7

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9DBR7

Rat Gene ID

116670

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116670

Rat Swissprot No.

Q10728

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q10728

免疫原

The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr853. AA range:621-670

特异性

Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

130kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosph

组织表达

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

细胞定位

Cytoplasm . Cytoplasm, cytoskeleton, stress fiber . Also along actomyosin filaments. .

信号通路

Vascular smooth muscle contraction;Focal adhesion;Long-term potentiation;Regulates Actin and Cytoskeleton;

功能

function:Regulates myosin phosphatase activity.,PTM:Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 6 ANK repeats.,subcellular location:Along actomyosin filaments and stress fibers.,subunit:PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 （Phospho-Thr853） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells, using MYPT1 （Phospho-Thr853） Antibody. The lane on the right is blocked with the phospho peptide.

Immunofluorescence analysis of human-lung tissue. 1,MYPT1 （phospho Thr853） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-heart tissue. 1,MYPT1 （phospho Thr853） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-kidney tissue. 1,MYPT1 （phospho Thr853） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of 293T cells using Phospho-MYPT1 （T853） Polyclonal Antibody diluted at 1：2000

Western Blot analysis of 293T AD293 22RV1 HELA cells using Phospho-MYPT1 （T853） Polyclonal Antibody diluted at 1：2000