BD-PP0654

IKKβ (phospho Tyr199) Rabbit Polyclonal Antibody

IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB

常规抗体

IKBKB

Inhibitor of nuclear factor kappa-B kinase subunit beta

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

3551

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3551

O14920

http://www.uniprot.org/uniprotkb/O14920/entry

16150

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16150

O88351

http://www.uniprot.org/uniprot/O88351

84351

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84351

Q9QY78

http://www.uniprot.org/uniprot/Q9QY78

The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr199. AA range:166-215

Phospho-IKKβ (Y199) Polyclonal Antibody detects endogenous levels of IKKβ protein only when phosphorylated at Y199.

WB 1:500-2000

85kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011],

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

Cytoplasm . Nucleus . Membrane raft . Colocalized with DPP4 in membrane rafts. .

MAPK_ERK_Growth;MAPK_G_Protein;Chemokine;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Toll_Like;NOD-like receptor;RIG-I-like receptor;Cytosolic DNA-sensing pathway;T_Cell_Receptor;B_Cell_Antigen;Neurotrophin;Insulin_Receptor;Adipocytoki

catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.,PTM:Ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues.,PTM:Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.,PTM:Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with Yersinia yopJ. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct.,tissue specificity:Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.