产品名称
Arrestin-β-1 (phospho Ser412) Rabbit Polyclonal Antibody
别名
ARRB1; ARR1; Beta-arrestin-1; Arrestin beta-1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=408
Human Swissprot No.
P49407
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P49407/entry
Mouse Swissprot No.
Q8BWG8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BWG8
免疫原
The antiserum was produced against synthesized peptide derived from human Arrestin 1 around the phosphorylation site of Ser412. AA range:369-418
特异性
Phospho-Arrestin-β-1 (S412) Polyclonal Antibody detects endogenous levels of Arrestin-β-1 protein only when phosphorylated at S412.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011],
组织表达
Brain,Peripheral blood,Uterus,
细胞定位
Cytoplasm. Nucleus. Cell membrane. Membrane, clathrin-coated pit . Cell projection, pseudopodium . Cytoplasmic vesicle. Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs. The monomeric form is predominantly located in the nucleus. The oligomeric form is located in the cytoplasm. Translocates to the nucleus upon stimulation of OPRD1 (By similarity). .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Chemokine;Endocytosis;
功能
function:Regulates beta-adrenergic receptor function. Beta-arrestins seem to bind phosphorylated beta-adrenergic receptors, thereby causing a significant impairment of their capacity to activate G(S) proteins.,online information:Arrestin entry,similarity:Belongs to the arrestin family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
