产品名称
FADD (phospho Ser194) Rabbit Polyclonal Antibody
别名
FADD; MORT1; GIG3; Protein FADD; FAS-associated death domain protein; FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8772
Human Swissprot No.
Q13158
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13158/entry
Mouse Swissprot No.
Q61160
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61160
免疫原
The antiserum was produced against synthesized peptide derived from human FADD around the phosphorylation site of Ser194. AA range:159-208
特异性
Phospho-FADD (S194) Polyclonal Antibody detects endogenous levels of FADD protein only when phosphorylated at S194.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008],
组织表达
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
细胞定位
cytoplasm,cytosol,plasma membrane,death-inducing signaling complex,CD95 death-inducing signaling complex,neuron projection,cell body,membrane raft,ripoptosome,
信号通路
Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Toll_Like;RIG-I-like receptor;Alzheimer's disease;Pathways in cancer;
功能
domain:Contains a death domain involved in the binding of the corresponding domain within Fas receptor.,function:Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.,PTM:Phosphorylated.,similarity:Contains 1 death domain.,similarity:Contains 1 DED (death effector) domain.,subunit:Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and LRDD.,tissue specificity:Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
