产品名称
Stat2 (phospho Tyr690) Rabbit Polyclonal Antibody
别名
STAT2; Signal transducer and activator of transcription 2; p113
蛋白名称
Signal transducer and activator of transcription 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6773
Human Swissprot No.
P52630
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P52630/entry
Mouse Swissprot No.
Q9WVL2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9WVL2
免疫原
The antiserum was produced against synthesized peptide derived from human STAT2 around the phosphorylation site of Tyr690. AA range:656-705
特异性
Phospho-Stat2 (Y690) Polyclonal Antibody detects endogenous levels of Stat2 protein only when phosphorylated at Y690.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010],
组织表达
Human small intestine,Lung,
细胞定位
Cytoplasm . Nucleus . Translocated into the nucleus upon activation by IFN-alpha/beta. .
功能
function:Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.,PTM:Tyrosine phosphorylated in response to IFN-alpha.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus upon activation by IFN-alpha/beta.,subunit:Interacts with ISGF3G/IRF-9 in the cytoplasm. Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation. Interacts with CRSP2 and CRSP6. Interacts with Simian virus 5 protein V and rabies virus phosphoprotein.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
