产品名称
Lyn (phospho Tyr508) Rabbit Polyclonal Antibody
别名
LYN; JTK8; Tyrosine-protein kinase Lyn; Lck/Yes-related novel protein tyrosine kinase; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn
蛋白名称
Tyrosine-protein kinase Lyn
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4067
Human Swissprot No.
P07948
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P07948/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17096
Mouse Swissprot No.
P25911
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P25911
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81515
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q07014
免疫原
The antiserum was produced against synthesized peptide derived from human Lyn around the phosphorylation site of Tyr507. AA range:463-512
特异性
Phospho-Lyn (Y508) Polyclonal Antibody detects endogenous levels of Lyn protein only when phosphorylated at Y508.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011],
组织表达
Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
细胞定位
Cell membrane. Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Membrane ; Lipid-anchor . Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. Detected on plasma membrane lipid rafts.
信号通路
Chemokine;B_Cell_Antigen;Fc epsilon RI;Fc gamma R-mediated phagocytosis;Long-term depression;Epithelial cell signaling in Helicobacter pylori infection;
功能
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with phosphorylated LIME1 and with CD79A upon BCR activation. Interacts with Epstein-Barr virus LMP2A. Interacts with TGFB1I1. Interaction, via the SH2 and SH3, domains with MUC1 is stimulated by IL7 and, the subsequent phosphorylation increases the binding between MUC1 and CTNNB1/beta-catenin. Interacts with PPP1R15A via the SH3 domain.,tissue specificity:Expressed in primary neuroblastoma tumors.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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