产品名称
eEF2K (phospho Ser366) Rabbit Polyclonal Antibody
别名
EEF2K; Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
蛋白名称
Eukaryotic elongation factor 2 kinase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29904
Human Swissprot No.
O00418
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O00418/entry
Mouse Swissprot No.
O08796
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O08796
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25435
Rat Swissprot Link
http://www.uniprot.org/uniprot/P70531
免疫原
The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366. AA range:331-380
特异性
Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008],
组织表达
Epithelium,Glial tumor,Lymph,T-cell,
细胞定位
cytoplasm,cytosol,postsynaptic density,
功能
catalytic activity:ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.,enzyme regulation:Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.,function:Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family.,similarity:Contains 1 alpha-type protein kinase domain.,subunit:Monomer or homodimer .,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
