产品名称
Synaptotagmin 1/2 (phospho Ser309/306) Rabbit Polyclonal Antibody
别名
SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT2; Synaptotagmin-2; Synaptotagmin II; SytII
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
6857/127833
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6857
Human Swissprot No.
P21579/Q8N9I0
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P21579/entry
Mouse Gene ID
20979/20980
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20979
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25716
Rat Swissprot No.
P21707/P29101
Rat Swissprot Link
http://www.uniprot.org/uniprot/P21707
免疫原
The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Ser309. AA range:276-325
特异性
Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody detects endogenous levels of Synaptotagmin 1/2 protein only when phosphorylated at S309/306.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],
组织表达
Expressed in melanocytes (PubMed:23999003).
细胞定位
Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass membrane protein . Cytoplasm .
功能
cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.,domain:The first C2 domain mediates Ca(2+)-dependent phospholipid binding.,domain:The second C2 domain mediates interaction with SV2A and STN2.,function:May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.,similarity:Belongs to the synaptotagmin family.,similarity:Contains 2 C2 domains.,subcellular location:Synaptic vesicles and chromaffin granules.,subunit:Homotetramer (Probable). Interacts with SCAMP5, STN2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
