BD-PP0585-Smad3 (phospho Ser425) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Smad3 (phospho Ser425) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0585

产品名称

Smad3 (phospho Ser425) Rabbit Polyclonal Antibody

别名

SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

类别

常规抗体

基因名称

SMAD3

蛋白名称

Mothers against decapentaplegic homolog 3

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4088

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4088

Human Swissprot No.

P84022

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P84022/entry

Mouse Gene ID

17127

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17127

Mouse Swissprot No.

Q8BUN5

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8BUN5

Rat Gene ID

25631

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25631

Rat Swissprot No.

P84025

Rat Swissprot Link

http://www.uniprot.org/uniprot/P84025

免疫原

The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425. AA range:376-425

特异性

Phospho-Smad3 (S425) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S425.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

58kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

组织表达

Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood

细胞定位

Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .

信号通路

Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;WNT;WNT-T CELLTGF-beta;Adherens_Junction;Pathways in cancer;Colorectal cancer;Pancreatic cancer;Chronic myeloid leukemia;

功能

disease:Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].,domain:The MH2 domain is sufficient to carry protein nuclear export.,function:Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with Smad4.,subunit:Interacts with HGS. Interacts with NEDD4L in response to TGF-beta. Interacts with TTRAP (By similarity). Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4. Interacts with JUN/FOS, vitamin D receptor, homeobox protein TGIF and TGIF2, PEBP2-alpha C subunit, CREB-binding protein (CBP), p300, SKI, SNON, ATF2, SMURF2, AIP1, DACH1 and TGFB1I1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Found in a complex with SMAD2 and TRIM33 upon addition of TGF-beta. Interacts with SMAD2 and TRIM33. Found in a complex with SMAD3, Ran and XPO4. Interacts with XPO4. Interacts with LBXCOR1 and CORL2.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using Smad3 （Phospho-Ser425） Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad3 （Phospho-Ser425） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with IGF-I 100ng/ml 10‘, using Smad3 （Phospho-Ser425） Antibody. The lane on the right is blocked with the phospho peptide.

Immunofluorescence analysis of rat-lung tissue. 1,Smad3 （phospho Ser425） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-kidney tissue. 1,Smad3 （phospho Ser425） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Smad3 （phospho Ser425） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Smad3 （phospho Ser425） Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Phospho-Smad3 （S425） Polyclonal Antibody diluted at 1：500

Western Blot analysis of HELA cells using Phospho-Smad3 （S425） Polyclonal Antibody diluted at 1：500