BD-PP0575-AMPKα1/2 (phospho Thr183/172) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AMPKα1/2 (phospho Thr183/172) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0575

产品名称

AMPKα1/2 (phospho Thr183/172) Rabbit Polyclonal Antibody

别名

PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;

类别

常规抗体

基因名称

AAPK1/AAPK2

蛋白名称

5'-AMP-activated protein kinase catalytic subunit alpha-1/2

推荐应用

反应种属

Human,Mouse,Rat,Monkey,Pig,Marsupenaeus japonicus

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5562/5563

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5562

Human Swissprot No.

Q13131/P54646

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q13131/entry

Mouse Gene ID

105787/108079

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=105787

Rat Gene ID

65248/78975

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=65248

Rat Swissprot No.

P54645/Q09137

Rat Swissprot Link

http://www.uniprot.org/uniprot/P54645

免疫原

The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

特异性

Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

63kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

组织表达

Brain,Intestine,Liver,Mammary gland,Platelet,Testis

细胞定位

Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .

信号通路

Insulin Receptor; mTOR; AMPK

功能

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using AMPK alpha （Phospho-Thr172） Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AMPK alpha （Phospho-Thr172） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells, using AMPK alpha （Phospho-Thr172） Antibody. The lane on the right is blocked with the phospho peptide.

Immunofluorescence analysis of rat-heart tissue. 1,AMPKα1/2 （phospho Thr183/172） Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of mouse-lung cells using primary antibody diluted at 1:1000（4°C overnight）. Secondary antibody：Goat Anti-rabbit IgG IRDye 800（ diluted at 1:5000, 25°C, 1 hour）. Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit（SM-005, Inventbiotech,MN,USA）.

Western Blot analysis of various cells using Phospho-AMPKα1/2 （T183/172） Polyclonal Antibody diluted at 1：500

Western Blot analysis of KB cells using Phospho-AMPKα1/2 （T183/172） Polyclonal Antibody diluted at 1：500

The picture was kindly provided by our customer

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.