产品名称
Syntaxin 1 (phospho Ser14) Rabbit Polyclonal Antibody
别名
STX1A; STX1; Syntaxin-1A; Neuron-specific antigen HPC-1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6804
Human Swissprot No.
Q16623
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q16623/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20907
Mouse Swissprot No.
O35526
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O35526
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116470
Rat Swissprot Link
http://www.uniprot.org/uniprot/P32851
免疫原
The antiserum was produced against synthesized peptide derived from human Syntaxin 1A around the phosphorylation site of Ser14. AA range:1-50
特异性
Phospho-Syntaxin 1 (S14) Polyclonal Antibody detects endogenous levels of Syntaxin 1 protein only when phosphorylated at S14.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],
组织表达
[Isoform 1]: Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex. ; [Isoform 2]: Expressed in heart, liver, fat, skeletal muscle, kidney and brain.
细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass type IV membrane protein . Cell junction, synapse, synaptosome . Cell membrane . Colocalizes with KCNB1 at the cell membrane. .; [Isoform 2]: Secreted .
信号通路
SNARE interactions in vesicular transport;
功能
disease:Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,function:Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis.,similarity:Belongs to the syntaxin family.,similarity:Contains 1 t-SNARE coiled-coil homology domain.,subunit:Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with OTOF (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU.,tissue specificity:Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
