产品名称
DAPK3 (phospho Thr265) Rabbit Polyclonal Antibody
别名
DAPK3; ZIPK; Death-associated protein kinase 3; DAP kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinase
蛋白名称
Death-associated protein kinase 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1613
Human Swissprot No.
O43293
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43293/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13144
Mouse Swissprot No.
O54784
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O54784
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64391
Rat Swissprot Link
http://www.uniprot.org/uniprot/O88764
免疫原
The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290
特异性
Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008],
组织表达
Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.
细胞定位
Nucleus . Cytoplasm . Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. .; [Isoform 1]: Nucleus . Cytoplasm .; [Isoform 2]: Nucleus . Cytoplasm .
信号通路
Pathways in cancer;Bladder cancer;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Associates to centromeres from prophase to anaphase.,subunit:Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF and CDC5L.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
