产品名称
Dok-1 (phospho Tyr398) Rabbit Polyclonal Antibody
别名
DOK1; Docking protein 1; Downstream of tyrosine kinase 1; p62(dok); pp62
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1796
Human Swissprot No.
Q99704
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q99704/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13448
Mouse Swissprot No.
P97465
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P97465
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=312477
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q4QQV2
免疫原
The antiserum was produced against synthesized peptide derived from human p62 Dok around the phosphorylation site of Tyr398. AA range:365-414
特异性
Phospho-Dok-1 (Y398) Polyclonal Antibody detects endogenous levels of Dok-1 protein only when phosphorylated at Y398.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
docking protein 1(DOK1) Homo sapiens The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016],
组织表达
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
细胞定位
[Isoform 1]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm, perinuclear region.
功能
domain:The PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.,PTM:Constitutively tyrosine-phosphorylated.,PTM:Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.,similarity:Belongs to the DOK family. Type A subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with ABL (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3.,tissue specificity:Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
