产品名称
CD31 (phospho Tyr713) Rabbit Polyclonal Antibody
别名
PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31
蛋白名称
Platelet endothelial cell adhesion molecule
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5175
Human Swissprot No.
P16284
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P16284/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18613
Mouse Swissprot No.
Q08481
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q08481
免疫原
The antiserum was produced against synthesized peptide derived from human PECAM-1 around the phosphorylation site of Tyr713. AA range:686-735
特异性
Phospho-CD31 (Y713) Polyclonal Antibody detects endogenous levels of CD31 protein only when phosphorylated at Y713.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010],
组织表达
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580308). Expressed on neutrophils (at protein level) (PubMed:17580308). Isoform Long predominates in all tissues examined (PubMed:12433657). Isoform Delta12 is detected only in trachea (PubMed:12433657). Isoform Delta14-15 is only detected in lung (PubMed:12433657). Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657). Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) (PubMed:12433657, PubMed:18388311).
细胞定位
Cell membrane ; Single-pass type I membrane protein . Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation. .; [Isoform Long]: Cell membrane ; Single-pass type I membrane protein . Membrane raft . Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. .; [Isoform Delta15]: Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
信号通路
Cell adhesion molecules (CAMs);Leukocyte transendothelial migration;
功能
function:This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.,online information:CD31 entry,online information:PECAM-1,online information:The Singapore human mutation and polymorphism database,PTM:Phosphorylated on Ser and Tyr residues after cellular activation.,similarity:Contains 6 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
