产品名称
FGFR-4 (phospho Tyr642) Rabbit Polyclonal Antibody
别名
FGFR4; JTK2; TKF; Fibroblast growth factor receptor 4; FGFR-4; CD antigen CD334
蛋白名称
Fibroblast growth factor receptor 4
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2264
Human Swissprot No.
P22455
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P22455/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14186
Mouse Swissprot No.
Q03142
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q03142
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25114
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q498D6
免疫原
Synthesized phospho-peptide around the phosphorylation site of human FGFR-4 (phospho Tyr642)
特异性
Phospho-FGFR-4 (Y642) Polyclonal Antibody detects endogenous levels of FGFR-4 protein only when phosphorylated at Y642.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of this gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgII
组织表达
Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
细胞定位
Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum. Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane.; [Isoform 2]: Secreted.; [Isoform 3]: Cytoplasm .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Endocytosis;Regulates Actin and Cytoskeleton;
功能
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.,PTM:Glycosylated (By similarity). Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 3 Ig-like C2-type (immunoglobulin-like) domains.,subcellular location:Isoform 2 may be secreted.,subunit:Interacts with KLB.,tissue specificity:Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
