产品名称
PC-PLD1 (phospho Thr147) Rabbit Polyclonal Antibody
别名
PLD1; Phospholipase D1; PLD 1; hPLD1; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5337
Human Swissprot No.
Q13393
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13393/entry
Mouse Swissprot No.
Q9Z280
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9Z280
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25096
Rat Swissprot Link
http://www.uniprot.org/uniprot/P70496
免疫原
Synthesized phospho-peptide around the phosphorylation site of human PC-PLD1 (phospho Thr147)
特异性
Phospho-PC-PLD1 (T147) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at T147.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011],
组织表达
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
细胞定位
Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Late endosome membrane ; Lipid-anchor ; Cytoplasmic side .
信号通路
Glycerophospholipid metabolism;Ether lipid metabolism;Endocytosis;Fc gamma R-mediated phagocytosis;GnRH;Pathways in cancer;Pancreatic cancer;
功能
catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,enzyme regulation:Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42. Inhibited by oleate.,function:Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.,online information:Phospholipase D entry,similarity:Belongs to the phospholipase D family.,similarity:Contains 1 PH domain.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 PLD phosphodiesterase domains.,subunit:Interacts with PIP5K1A.,tissue specificity:Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
