产品名称
Zyxin (phospho Ser142) Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7791
Human Swissprot No.
Q15942
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15942/entry
Mouse Swissprot No.
Q62523
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q62523
免疫原
The antiserum was produced against synthesized peptide derived from human Zyxin around the phosphorylation site of Ser142. AA range:108-157
特异性
Phospho-Zyxin (S142) Polyclonal Antibody detects endogenous levels of Zyxin protein only when phosphorylated at S142.
稀释度
WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008],
组织表达
Cervix carcinoma,Epididymis,Epithelium,Kidney,Placenta,Platelet,Skin,Umbilical vein,Uterus,
细胞定位
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.
功能
function:Adhesion plaque protein. Binds alpha-actinin and the CRP protein. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.,similarity:Belongs to the zyxin/ajuba family.,similarity:Contains 3 LIM zinc-binding domains.,subcellular location:Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.,subunit:Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH and VASP. Interaction with ENA/VASP family members is important for their targeting to focal adhesions and the formation of actin-rich structures.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
