BD-PP0469

Eps15 (phospho Tyr849) Rabbit Polyclonal Antibody

EPS15; AF1P; Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p

常规抗体

EPS15

Epidermal growth factor receptor substrate 15

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

2060

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2060

P42566

http://www.uniprot.org/uniprotkb/P42566/entry

13858

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13858

P42567

http://www.uniprot.org/uniprot/P42567

Synthesized phospho-peptide around the phosphorylation site of human Eps15 (phospho Tyr849)

Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849.

WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

100kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009],

Ubiquitously expressed.

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes. .; [Isoform 2]: Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Colocalizes with HGS on bilayered clathrin coats on endosomes.

Endocytosis;

disease:A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with MLL/HRX. The result is a rogue activator protein.,domain:The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.,function:Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits.,PTM:Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.,similarity:Contains 2 EF-hand domains.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,similarity:Contains 3 EH domains.,subcellular location:Colocalizes with HGS on bilayered clathrin coats on endosomes.,subcellular location:Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.,subunit:Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM1 or STAM2. Binds AP2A2 and AP2B1. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP.,tissue specificity:Ubiquitously expressed.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.