BD-PP0451

c-Src (phospho Tyr216) Rabbit Polyclonal Antibody

SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

常规抗体

SRC

Proto-oncogene tyrosine-protein kinase Src

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

6714

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6714

P12931

http://www.uniprot.org/uniprotkb/P12931/entry

20779

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20779

P05480

http://www.uniprot.org/uniprot/P05480

Q9WUD9

http://www.uniprot.org/uniprot/Q9WUD9

The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr216. AA range:181-230

Phospho-c-Src (Y216) Polyclonal Antibody detects endogenous levels of c-Src protein only when phosphorylated at Y216.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

50kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.; [Isoform 1]: Expressed in spleen and liver. ; [Isoform 2]: Expressed in brain. ; [Isoform 3]: Expressed in brain.

Cell membrane ; Lipid-anchor . Mitochondrion inner membrane . Nucleus . Cytoplasm, cytoskeleton . Cytoplasm, perinuclear region . Cell junction, focal adhesion . Localizes to focal adhesion sites following integrin engagement (PubMed:22801373). Localization to focal adhesion sites requires myristoylation and the SH3 domain (PubMed:7525268). Colocalizes with PDLIM4 at the perinuclear region, but not at focal adhesions (PubMed:19307596). .

ErbB_HER;Endocytosis;VEGF;Focal adhesion;Adherens_Junction;Adherens_Junction;Gap junction;GnRH;Epithelial cell signaling in Helicobacter pylori infection;

catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,PTM:Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.