产品名称
Dynamin I (phospho Ser778) Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1759
Human Swissprot No.
Q05193
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q05193/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13429
Mouse Swissprot No.
P39053
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P39053
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=140694
Rat Swissprot Link
http://www.uniprot.org/uniprot/P21575
免疫原
The antiserum was produced against synthesized peptide derived from human DYN1 around the phosphorylation site of Ser778. AA range:751-800
特异性
Phospho-Dynamin I (S778) Polyclonal Antibody detects endogenous levels of Dynamin I protein only when phosphorylated at S778.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
dynamin 1(DNM1) Homo sapiens This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton . Microtubule-associated.
信号通路
Endocytosis;Fc gamma R-mediated phagocytosis;
功能
catalytic activity:GTP + H(2)O = GDP + phosphate.,function:Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.,similarity:Belongs to the dynamin family.,similarity:Contains 1 GED domain.,similarity:Contains 1 PH domain.,subcellular location:Microtubule-associated.,subunit:Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
