产品名称
PDGFR-α (phospho Tyr849) Rabbit Polyclonal Antibody
别名
PDGFRA; PDGFR2; RHEPDGFRA; Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD14
蛋白名称
Platelet-derived growth factor receptor alpha
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5156
Human Swissprot No.
P16234
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P16234/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18595
Mouse Swissprot No.
P26618
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P26618
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25267
Rat Swissprot Link
http://www.uniprot.org/uniprot/P20786
免疫原
The antiserum was produced against synthesized peptide derived from human PDGFRa around the phosphorylation site of Tyr849. AA range:816-865
特异性
Phospho-PDGFR-α (Y849) Polyclonal Antibody detects endogenous levels of PDGFR-α protein only when phosphorylated at Y849.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012],
组织表达
Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
细胞定位
Cell membrane ; Single-pass type I membrane protein . Cell projection, cilium . Golgi apparatus .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Calcium;Cytokine-cytokine receptor interaction;Endocytosis;Focal adhesion;Gap junction;Regulates Actin and Cytoskeleton;Pathways in cancer;Colorectal cancer;Glioma;Prostate cancer;Melanoma;
功能
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:A fusion of PDGFRA and FIP1L1 (FIP1L1-PDGFRA), due to an interstitial chromosomal deletion, is the cause of some cases of hypereosinophilic syndrome (HES) [MIM:607685]. HES is a rare hematologic disorder characterized by sustained overproduction of eosinophils in the bone marrow, eosinophilia, tissue infiltration and organ damage.,function:Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Homodimer, and heterodimer with PDGFRB. Interacts with the SH2 domain of SHB via phosphorylated Tyr-720 (By similarity). Interacts with the SH2 domain of SHF via phosphorylated Tyr-720.,tissue specificity:Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
