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Myf-5 (phospho Ser49) Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PP0395
产品名称
Myf-5 (phospho Ser49) Rabbit Polyclonal Antibody
别名
MYF5; BHLHC2; Myogenic factor 5; Myf-5; Class C basic helix-loop-helix protein 2; bHLHc2
类别
常规抗体
基因名称
MYF5
蛋白名称
Myogenic factor 5
推荐应用
WB
反应种属
Human,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
4617
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4617
Human Swissprot No.
P13349
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P13349/entry
Mouse Gene ID
17877
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17877
Mouse Swissprot No.
P24699
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P24699
免疫原
The antiserum was produced against synthesized peptide derived from human Myogenic Factor 5 around the phosphorylation site of Ser49. AA range:21-70
特异性
Phospho-Myf-5 (S49) Polyclonal Antibody detects endogenous levels of Myf-5 protein only when phosphorylated at S49.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
预测分子量
38kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
组织表达
Skeletal muscle,
细胞定位
Nucleus .
功能
function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HeLa cells, using Myogenic Factor 5 (Phospho-Ser49) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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