BD-PP0390

Wee1 (phospho Ser642) Rabbit Polyclonal Antibody

WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase

常规抗体

WEE1

Wee1-like protein kinase

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

7465

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7465

P30291

http://www.uniprot.org/uniprotkb/P30291/entry

22390

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22390

P47810

http://www.uniprot.org/uniprot/P47810

308937

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=308937

Q63802

http://www.uniprot.org/uniprot/Q63802

The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser642. AA range:597-646

Phospho-Wee1 (S642) Polyclonal Antibody detects endogenous levels of Wee1 protein only when phosphorylated at S642.

WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

100kD

-20°C/1 year

Polyclonal, Rabbit,IgG

WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],

Amygdala,Blood,Epithelium,Human uterus endothel primary cell culture,Placenta,Skin,

Nucleus.

Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;

catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 2 magnesium ions per subunit.,enzyme regulation:Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.,function:May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDC2 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDC2 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDC2 does not occur.,PTM:Phosphorylated during M and G1 phases. Also autophosphorylated.,PTM:Ubiquitinated and degraded at the onset of G2/M phase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.,similarity:Contains 1 protein kinase domain.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.