产品名称
C3G (phospho Tyr504) Rabbit Polyclonal Antibody
别名
RAPGEF1; GRF2; Rap guanine nucleotide exchange factor 1; CRK SH3-binding GNRP; Guanine nucleotide-releasing factor 2; Protein C3G
蛋白名称
Rap guanine nucleotide exchange factor 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2889
Human Swissprot No.
Q13905
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13905/entry
免疫原
The antiserum was produced against synthesized peptide derived from human RapGEF1 around the phosphorylation site of Tyr504. AA range:470-519
特异性
Phospho-C3G (Y504) Polyclonal Antibody detects endogenous levels of C3G protein only when phosphorylated at Y504.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008],
组织表达
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
信号通路
Regulation of Actin Dynamics; AMPK
功能
function:Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,subunit:Interacts with CRK via its SH3-binding sites.,tissue specificity:Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
