BD-PP0366-HBP1 (phospho Ser402) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HBP1 (phospho Ser402) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0366

产品名称

HBP1 (phospho Ser402) Rabbit Polyclonal Antibody

别名

HBP1; HMG box-containing protein 1; HMG box transcription factor 1; High mobility group box transcription factor 1

类别

常规抗体

基因名称

HBP1

蛋白名称

HMG box-containing protein 1

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

26959

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26959

Human Swissprot No.

O60381

Human Swissprot Link

http://www.uniprot.org/uniprotkb/O60381/entry

Mouse Swissprot No.

Q8R316

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8R316

免疫原

The antiserum was produced against synthesized peptide derived from human HBP1 around the phosphorylation site of Ser402. AA range:371-420

特异性

Phospho-HBP1 (S402) Polyclonal Antibody detects endogenous levels of HBP1 protein only when phosphorylated at S402.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

预测分子量

58kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

function:Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.,similarity:Contains 1 AXH domain.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the second PAH repeat of SIN3A (By similarity). Binds TCF4 and RB1.,

组织表达

Brain,Hepatoma,Uterus,

细胞定位

Nucleus .

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30‘, using HBP1 （Phospho-Ser402） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of A549 cells using Phospho-HBP1 （S402） Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.