产品名称
MCM4 (phospho Ser54) Rabbit Polyclonal Antibody
别名
MCM4; CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21
蛋白名称
DNA replication licensing factor MCM4
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4173
Human Swissprot No.
P33991
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P33991/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17217
Mouse Swissprot No.
P49717
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P49717
免疫原
The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54. AA range:20-69
特异性
Phospho-MCM4 (S54) Polyclonal Antibody detects endogenous levels of MCM4 protein only when phosphorylated at S54.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcri
组织表达
Cervix,Epithelium,Testis,
细胞定位
Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .
信号通路
DNA replication;Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;
功能
function:Involved in the control of DNA replication.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
