产品名称
NUF2 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/83540
Human Swissprot No.
Q9BZD4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9BZD4/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/66977
Mouse Swissprot No.
Q99P69
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q99P69
Rat Gene Link
https://www.uniprot.org/uniprot/304951
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q6AYL9
免疫原
Synthesized peptide derived from human NUF2 AA range: 199-249
特异性
This antibody detects endogenous levels of NUF2 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008],
细胞定位
Nucleus. Chromosome, centromere, kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization.
功能
function:Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.,PTM:Can be phosphorylated by AURKA and AURKB.,similarity:Belongs to the NUF2 family.,subcellular location:Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.,subunit:Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
