产品名称
Csk (phospho Ser364) Rabbit Polyclonal Antibody
别名
CSK; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL
蛋白名称
Tyrosine-protein kinase CSK
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1445
Human Swissprot No.
P41240
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P41240/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12988
Mouse Swissprot No.
P41241
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P41241
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=315707
Rat Swissprot Link
http://www.uniprot.org/uniprot/P32577
免疫原
The antiserum was produced against synthesized peptide derived from human Csk around the phosphorylation site of Ser364. AA range:330-379
特异性
Phospho-Csk (S364) Polyclonal Antibody detects endogenous levels of Csk protein only when phosphorylated at S364.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.,PTM:Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Mainly cytoplasmic, also present in lipid rafts.,subunit:Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.,tissue specificity:Expressed in lung and macrophages.,
组织表达
Expressed in lung and macrophages.
细胞定位
Cytoplasm . Cell membrane . Mainly cytoplasmic, also present in lipid rafts. .
信号通路
Chemokine;Neurotrophin;Regulates Actin and Cytoskeleton;Epithelial cell signaling in Helicobacter pylori infection;
功能
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.,PTM:Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Mainly cytoplasmic, also present in lipid rafts.,subunit:Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.,tissue specificity:Expressed in lung and macrophages.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
