BD-PP0280-eIF4B (phospho Ser422) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

eIF4B (phospho Ser422) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0280

产品名称

eIF4B (phospho Ser422) Rabbit Polyclonal Antibody

别名

EIF4B; Eukaryotic translation initiation factor 4B; eIF-4B

类别

常规抗体

基因名称

EIF4B

蛋白名称

Eukaryotic translation initiation factor 4B

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3716

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3716

Human Swissprot No.

P23588

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P23588/entry

Mouse Gene ID

75705

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=75705

Mouse Swissprot No.

Q8BGD9

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8BGD9

免疫原

The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422. AA range:388-437

特异性

Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

80kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,

组织表达

Adipose tissue,Brain,Cervix carcinoma,Epithelium,Liver,Lung

细胞定位

cytosol,polysome,eukaryotic translation initiation factor 4F complex,dendrite,neuronal cell body,

信号通路

mTOR;

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using eIF4B （Phospho-Ser422） Antibody

Immunofluorescence analysis of NIH/3T3 cells, using eIF4B （Phospho-Ser422） Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using eIF4B （Phospho-Ser422） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells, using eIF4B （Phospho-Ser422） Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of 293 cells using Phospho-eIF4B （S422） Polyclonal Antibody diluted at 1：2000