产品名称
Vav (phospho Tyr174) Rabbit Polyclonal Antibody
别名
VAV1; VAV; Proto-oncogene vav
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7409
Human Swissprot No.
P15498
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P15498/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22324
Mouse Swissprot No.
P27870
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P27870
Rat Swissprot Link
http://www.uniprot.org/uniprot/P54100
免疫原
Synthesized phospho-peptide around the phosphorylation site of human Vav (phospho Tyr174)
特异性
Phospho-Vav (Y174) Polyclonal Antibody detects endogenous levels of Vav protein only when phosphorylated at Y174.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012],
组织表达
Widely expressed in hematopoietic cells but not in other cell types.
细胞定位
intracellular,cytosol,plasma membrane,cell-cell junction,
信号通路
Chemokine;Focal adhesion;Natural killer cell mediated cytotoxicity;T_Cell_Receptor;B_Cell_Antigen;Fc epsilon RI;Fc gamma R-mediated phagocytosis;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton;
功能
domain:The DH domain is involved in interaction with CCPG1.,function:Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.,miscellaneous:'Vav' stands for the sixth letter of the Hebrew alphabet.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:May interact with CCPG1 (By similarity). Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK.,tissue specificity:Widely expressed in hematopoietic cells but not in other cell types.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
