产品名称
Stat6 (phospho Thr645) Rabbit Polyclonal Antibody
别名
STAT6; Signal transducer and activator of transcription 6; IL-4 Stat
蛋白名称
Signal transducer and activator of transcription 6
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6778
Human Swissprot No.
P42226
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P42226/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20852
Mouse Swissprot No.
P52633
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P52633
免疫原
The antiserum was produced against synthesized peptide derived from human STAT6 around the phosphorylation site of Thr645. AA range:612-661
特异性
Phospho-Stat6 (T645) Polyclonal Antibody detects endogenous levels of Stat6 protein only when phosphorylated at T645.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
功能
function:Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.,PTM:Tyrosine phosphorylated following stimulation by IL-4 and IL-3.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member (By similarity). Interacts with NCOA1 via its C-terminal LXXLL motif.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
