产品名称
SPC25 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/57405
Human Swissprot No.
Q9HBM1
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9HBM1/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/66442
Mouse Swissprot No.
Q3UA16
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q3UA16
Rat Gene Link
https://www.uniprot.org/uniprot/295661
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q5M856
免疫原
Synthesized peptide derived from human SPC25 AA range: 17-67
特异性
This antibody detects endogenous levels of SPC25 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. [provided by RefSeq, Jul 2008],
细胞定位
Nucleus. Chromosome, centromere, kinetochore . Localizes to kinetochores from late prophase to anaphase (PubMed:14738735). Localizes specifically to the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129). .
功能
function:Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.,similarity:Belongs to the SPC25 family.,subcellular location:Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.,subunit:Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
