产品名称
p21 (phospho Thr145) Rabbit Polyclonal Antibody
别名
CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
蛋白名称
Cyclin-dependent kinase inhibitor 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1026
Human Swissprot No.
P38936
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P38936/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12575
Mouse Swissprot No.
P39689
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P39689
免疫原
The antiserum was produced against synthesized peptide derived from human p21 Cip1 around the phosphorylation site of Thr145. AA range:111-160
特异性
Phospho-p21 (T145) Polyclonal Antibody detects endogenous levels of p21 protein only when phosphorylated at T145.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lac
组织表达
Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
细胞定位
Cytoplasm . Nucleus .
信号通路
Stem cell pathway; ErbB/HER; PI3K/Akt; AMPK; Cell_Cycle_G1S;Cell_Cycle_G2M_DNA; Protein_Acetylation
功能
function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
