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Jun B (phospho Ser259) Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PP0158
产品名称
Jun B (phospho Ser259) Rabbit Polyclonal Antibody
别名
JUNB; Transcription factor jun-B
类别
常规抗体
基因名称
JUNB
蛋白名称
Transcription factor jun-B
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
3726
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3726
Human Swissprot No.
P17275
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P17275/entry
Mouse Gene ID
16477
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16477
Mouse Swissprot No.
P09450
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P09450
Rat Gene ID
24517
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24517
Rat Swissprot No.
P24898
Rat Swissprot Link
http://www.uniprot.org/uniprot/P24898
免疫原
The antiserum was produced against synthesized peptide derived from human JunB around the phosphorylation site of Ser259. AA range:226-275
特异性
Phospho-Jun B (S259) Polyclonal Antibody detects endogenous levels of Jun B protein only when phosphorylated at S259.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. ELISA: 1:20000.. IF 1:50-200
预测分子量
33kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.,induction:By growth factors.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as an homodimer or as an heterodimer with another member of the jun/fos family.,
组织表达
Adipose tissue,Epithelium,Pancreas,Placenta,Prostat
细胞定位
Nucleus.
功能
function:Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.,induction:By growth factors.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as an homodimer or as an heterodimer with another member of the jun/fos family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser259) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells, using JunB (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Jun B (S259) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

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