产品名称
Flk-1 (phospho Tyr1175) Rabbit Polyclonal Antibody
别名
KDR; FLK1; VEGFR2; Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD antigen CD309
蛋白名称
Vascular endothelial growth factor receptor 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3791
Human Swissprot No.
P35968
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P35968/entry
Mouse Swissprot No.
P35918
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P35918
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25589
Rat Swissprot Link
http://www.uniprot.org/uniprot/O08775
免疫原
The antiserum was produced against synthesized peptide derived from human VEGFR2 around the phosphorylation site of Tyr1175. AA range:1141-1190
特异性
Phospho-Flk-1 (Y1175) Polyclonal Antibody detects endogenous levels of Flk-1 protein only when phosphorylated at Y1175.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009],
组织表达
Detected in cornea (at protein level). Widely expressed.
细胞定位
Cell junction . Endoplasmic reticulum . Cell membrane . Localized with RAP1A at cell-cell junctions (By similarity). Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610). .; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.; [Isoform 2]: Secreted .; [Isoform 3]: Secreted.
信号通路
Cytokine-cytokine receptor interaction;Endocytosis;VEGF;Focal adhesion;
功能
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 7 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Interacts with MYOF (By similarity). Interacts with SHB; upon VEGF activation. Interacts with HIV-1 Tat.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
