产品名称
Erbin (phospho Tyr1104) Rabbit Polyclonal Antibody
别名
ERBB2IP; ERBIN; KIAA1225; LAP2; Protein LAP2; Densin-180-like protein; Erbb2-interacting protein; Erbin
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55914
Human Swissprot No.
Q96RT1
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q96RT1/entry
Mouse Swissprot No.
Q80TH2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q80TH2
免疫原
Synthesized phospho-peptide around the phosphorylation site of human Erbin (phospho Tyr1104)
特异性
Phospho-Erbin (Y1104) Polyclonal Antibody detects endogenous levels of Erbin protein only when phosphorylated at Y1104.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a member of the leucine-rich repeat and PDZ domain (LAP) family. The encoded protein contains 17 leucine-rich repeats and one PDZ domain. It binds to the unphosphorylated form of the ERBB2 protein and regulates ERBB2 function and localization. It has also been shown to affect the Ras signaling pathway by disrupting Ras-Raf interaction. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
组织表达
Highly expressed in brain, heart, kidney, muscle and stomach, followed by liver, spleen and intestine.
细胞定位
Cell junction, hemidesmosome . Nucleus membrane . Basolateral cell membrane . Found in hemidesmosomes, which are cell-substrate adhesion complexes in stratified epithelia. In transfected cells, either diffusely distributed over the cytoplasm or concentrated at the basolateral membrane. Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes (By similarity). .
功能
function:Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the LAP (LRR and PDZ) protein family.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 17 LRR (leucine-rich) repeats.,subcellular location:Found in hemidesmosomes, which are cell-substrate adhesion complexes in stratified epithelia. In transfected cells, either diffusely distributed over the cytoplasm or concentrated at the basolateral membrane.,subunit:Interacts with ERBB2, BPAG1 and ITGB4. May favor the localization of ERBB2, by restricting its presence to the basolateral membrane of epithelial cells. Also found to interact with ARVCF and delta catenin.,tissue specificity:Highly expressed in brain, heart, kidney, muscle and stomach, followed by liver, spleen and intestine.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
