产品名称
Elk-1 (phospho Ser389) Rabbit Polyclonal Antibody
别名
ELK1; ETS domain-containing protein Elk-1
蛋白名称
ETS domain-containing protein Elk-1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2002
Human Swissprot No.
P19419
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P19419/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13712
Mouse Swissprot No.
P41969
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P41969
免疫原
The antiserum was produced against synthesized peptide derived from human Elk1 around the phosphorylation site of Ser389. AA range:356-405
特异性
Phospho-Elk-1 (S389) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at S389.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012],
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;ErbB_HER;Focal adhesion;Insulin_Receptor;GnRH;Prion diseases;Endometrial cancer;
功能
function:Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.,PTM:On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.,PTM:Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity.,tissue specificity:Lung and testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
