BD-PP0051

CD3 ζ (phospho Tyr142) Rabbit Polyclonal Antibody

CD247; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247

常规抗体

CD247

T-cell surface glycoprotein CD3 zeta chain

WB

Human,Mouse,Rat,Monkey

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

919

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=919

P20963

http://www.uniprot.org/uniprotkb/P20963/entry

12503

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12503

P24161

http://www.uniprot.org/uniprot/P24161

The antiserum was produced against synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. AA range:111-160

Phospho-CD3 ζ (Y142) Polyclonal Antibody detects endogenous levels of CD3 ζ protein only when phosphorylated at Y142.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

20kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641).

Cell membrane ; Single-pass type I membrane protein.

Natural killer cell mediated cytotoxicity;T_Cell_Receptor;

disease:Defects in CD247 are a cause of primary T-cell immunodeficiency [MIM:610163]. Affected individuals suffer of recurrent infections. Patients T-cell counts are very low and B-cell counts are normal.,domain:The ITAM domains mediate interaction with SHB.,function:Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.,online information:CD247 mutation db,PTM:Phosphorylated on Tyr residues after T-cell receptor triggering.,similarity:Belongs to the CD3Z/FCER1G family.,similarity:Contains 3 ITAM domains.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts with DOCK2 and TRAT1. Interacts with HIV-1 Nef protein. Interacts with SHB.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.