产品名称
CaMKIV (phospho Thr200) Rabbit Polyclonal Antibody
别名
CAMK4; CAMK; CAMK-GR; CAMKIV; Calcium/calmodulin-dependent protein kinase type IV; CaMK IV; CaM kinase-GR
蛋白名称
Calcium/calmodulin-dependent protein kinase type IV
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=814
Human Swissprot No.
Q16566
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q16566/entry
Mouse Swissprot No.
P08414
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P08414
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25050
Rat Swissprot Link
http://www.uniprot.org/uniprot/P13234
免疫原
The antiserum was produced against synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr196/200. AA range:166-215
特异性
Phospho-CaMKIV (T200) Polyclonal Antibody detects endogenous levels of CaMKIV protein only when phosphorylated at T200.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008],
组织表达
Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.
细胞定位
Cytoplasm. Nucleus. Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity). .
信号通路
Calcium;Long-term potentiation;Neurotrophin;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca(2+)/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca(2+)/calmodulin-independent state (By similarity). Probably inactivated by serine/threonine protein phosphatase 2A.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.,PTM:Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.,subunit:Monomer (By similarity). Interacts with serine/threonine protein phosphatase 2A catalytic subunit, PPP2CA or PPP2CB. The interaction with PP2CA or PP2CB is mutually exclusive with binding to Ca(2+)/calmodulin.,tissue specificity:Expressed in epithelial ovarian cancer tissue.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
