产品名称
AMPKα1 (phospho Ser496) Rabbit Polyclonal Antibody
别名
PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1
蛋白名称
5'-AMP-activated protein kinase catalytic subunit alpha-1
反应种属
Human,Mouse,Rat,Canine,Fish
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5562
Human Swissprot No.
Q13131
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13131/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=105787
Mouse Swissprot No.
Q5EG47
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q5EG47
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=65248
Rat Swissprot Link
http://www.uniprot.org/uniprot/P54645
免疫原
The antiserum was produced against synthesized peptide derived from human AMPK1 around the phosphorylation site of Ser496. AA range:451-500
特异性
Phospho-AMPKα1 (S496) Polyclonal Antibody detects endogenous levels of AMPKα1 protein only when phosphorylated at S496.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
组织表达
Brain,Intestine,Liver,Mammary gland,Platelet,Testis
细胞定位
Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. .
信号通路
Insulin Receptor; mTOR; AMPK
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
