BD-PP0003-4E-BP1 (phospho Thr69) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

4E-BP1 (phospho Thr69) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PP0003

产品名称

4E-BP1 (phospho Thr69) Rabbit Polyclonal Antibody

别名

EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

类别

常规抗体

基因名称

EIF4EBP1

蛋白名称

Eukaryotic translation initiation factor 4E-binding protein 1

推荐应用

反应种属

Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Mouse Gene ID

13685

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13685

Rat Gene ID

116636

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116636

Rat Swissprot No.

Q62622

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q62622

免疫原

The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69. AA range:35-84

特异性

Phospho-4E-BP1 (T69) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T69.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

12kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

EIF4EBP1 encodes one member of a family of translation repressor proteins. The eukaryotic translation initiation factor 4E binding protein 1 directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using 4E-BP1 （Phospho-Thr69） Antibody

Immunohistochemistry analysis of paraffin-embedded human brain carcinoma, using 4E-BP1 （Phospho-Thr69） Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30‘, using 4E-BP1 （Phospho-Thr69） Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.