产品名称
TRYB1 Rabbit Polyclonal Antibody
基因名称
TPSAB1 TPS1 TPS2 TPSB1
蛋白名称
Tryptase alpha/beta-1 (Tryptase-1) (EC 3.4.21.59) (Tryptase I) (Tryptase alpha-1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q15661
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q15661/entry
Mouse Swissprot No.
Q02844
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q02844
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P27435
免疫原
Synthesized peptide derived from part region of human protein AA range: 200-250
特异性
TRYB1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate gene
组织表达
Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level).
细胞定位
Secreted. Released from the secretory granules upon mast cell activation. .
功能
catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.,function:Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.,function:Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection.,polymorphism:There are two alleles; beta-II and beta-III which differ by 3 residues.,similarity:Belongs to the peptidase S1 family.,similarity:Belongs to the peptidase S1 family. Tryptase subfamily.,similarity:Contains 1 peptidase S1 domain.,subcellular location:Released from the secretory granules upon mast cell activation.,subunit:Homotetramer.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
