产品名称
ILF2 Rabbit Polyclonal Antibody
蛋白名称
Interleukin enhancer-binding factor 2 (Nuclear factor of activated T-cells 45 kDa)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q12905
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q12905/entry
Mouse Swissprot No.
Q9CXY6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9CXY6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q7TP98
免疫原
Synthesized peptide derived from part region of human protein
特异性
ILF2 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014],
组织表达
Fetal liver,Lung,Thymus,
细胞定位
Nucleus, nucleolus. Cytoplasm . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
功能
function:Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Chimeric cDNA.,similarity:Contains 1 DZF domain.,subunit:Forms heterodimers with ILF3. ILF2-ILF3 heterodimers may also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of G22P1/KU70 and XRCC5/KU80.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
