产品名称
KLOT Rabbit Polyclonal Antibody
蛋白名称
Klotho (EC 3.2.1.31) [Cleaved into: Klotho peptide]
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9UEF7
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9UEF7/entry
Mouse Swissprot No.
O35082
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O35082
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q9Z2Y9
免疫原
Synthesized peptide derived from part region of human protein AA range: 400-500
特异性
KLOT Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008],
组织表达
Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
细胞定位
[Isoform 1]: Cell membrane ; Single-pass type I membrane protein . Apical cell membrane ; Single-pass type I membrane protein . Isoform 1 shedding leads to a soluble peptide. .; [Isoform 2]: Secreted .; [Klotho peptide]: Secreted .
功能
catalytic activity:A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol.,disease:Defects in KL may be a cause of chronic renal failure complications.,domain:Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.,function:May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D.,function:The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.,online information:The thread of life -Issue 65 of December 2005,polymorphism:Homozygosity for KL-VS allele is associated with decreased longevity and increased cardiovascular disease risk.,PTM:N-glycosylated.,similarity:Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.,subcellular location:Isoform 1 shedding leads to a soluble peptide.,subunit:Homodimer.,tissue specificity:Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
