产品名称
SYEP Rabbit Polyclonal Antibody
基因名称
EPRS GLNS PARS QARS QPRS PIG32
蛋白名称
Bifunctional glutamate/proline--tRNA ligase (Bifunctional aminoacyl-tRNA synthetase) (Cell proliferation-inducing gene 32 protein) [Includes: Glutamate--tRNA ligase (EC 6.1.1.17) (Glutamyl-tRNA synthetase) (GluRS); Proline--tRNA ligase (EC 6.1.1.15) (Prolyl-tRNA synthetase)]
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P07814
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P07814/entry
Mouse Swissprot No.
Q8CGC7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8CGC7
免疫原
Synthesized peptide derived from part region of human protein
特异性
SYEP Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008],
组织表达
Bone marrow,Brain,Cervix carcinoma,Duodenum,Epithelium,Eye,Lung,Placenta,
细胞定位
Cytoplasm, cytosol . Membrane ; Peripheral membrane protein . Translocates from cytosol to membranes upon phosphorylation at Ser-999. .
信号通路
Porphyrin and chlorophyll metabolism;Aminoacyl-tRNA biosynthesis;
功能
catalytic activity:ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).,catalytic activity:ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).,caution:Was originally thought to be a glutaminyl-tRNA synthetase.,domain:The WHEP-TRS domain is involved in RNA binding.,function:Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.,sequence caution:Contaminating sequence. Potential poly-A sequence.,sequence caution:Sequencing errors.,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,similarity:Contains 3 WHEP-TRS domains.,similarity:In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.,similarity:In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.,subunit:Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with DUS2L.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
