产品名称
IF5A1 Rabbit Polyclonal Antibody
蛋白名称
Eukaryotic translation initiation factor 5A-1 (eIF-5A-1) (eIF-5A1) (Eukaryotic initiation factor 5A isoform 1) (eIF-5A) (Rev-binding factor) (eIF-4D)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P63241
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P63241/entry
Mouse Swissprot No.
P63242
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P63242
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q3T1J1
免疫原
Synthesized peptide derived from part region of human protein
特异性
IF5A1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.,tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,
组织表达
Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
细胞定位
Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (PubMed:19379712, PubMed:27306458). Nuclear export of hypusinated protein is mediated by XPO4 (PubMed:10944119, PubMed:27306458). .
功能
function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,subunit:Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.,tissue specificity:Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
